Table 2.

Primers for KASP and Sanger sequencing

Primers	Primer sequence (5′-3′)
1. Sequencing primer-Forward	AACAACTGACCCCGGTGGCG
2. Sequencing primer-Reverse	ATGGCGCTGAGGCCGCGCTCGG
3. RS429358-primer-FAM	CGCGGACATGGAGGACGTGT
4. RS429358-primer-HEX	GCGGACATGGAGGACGTGC
5. RS429358-primer-Common	CTCGCCGCGGTACTGCACC
6. RS7412-primer-FAM	GATGCCGATGACCTGCAGAAGT
7. RS7412-primer-HEX	ATGCCGATGACCTGCAGAAGC
8. RS7412-primer-Common	CCCGGCCTGGTACACTGCC

Primers “1, 2” for Sanger sequencing, “3, 4, 5” for KASP in rs429358 genotyping, and “6, 7, 8” for KASP in rs7412 genotyping