Table 2.
Top Replicated Cytosine-Phosphate-Guanine Site Associations Between Blood DNA Methylation Levels and Number of Drinks per Week in the Past Year, Sister Study, United States, 2004–2015
| CpG Site | Gene | Chromosome | Discovery Set (n = 2,775) | Replication Set (n = 187) | |||
|---|---|---|---|---|---|---|---|
| % Methylated | Point Estimatea | Q Value | Point Estimatea | P Value | |||
| cg14476101 | PHGDH | 1 | 74.9 | −0.015 | 2 × 10−18 | −0.027 | 5 × 10−11 |
| cg11376147 | SLC43A1 | 11 | 12 | −0.007 | 2 × 10−15 | −0.012 | 2 × 10−4 |
| cg06690548 | SLC7A11 | 4 | 95.4 | −0.016 | 2 × 10−15 | −0.019 | 7 × 10−5 |
| cg16246545 | PHGDH | 1 | 55 | −0.009 | 5 × 10−12 | −0.017 | 1 × 10−6 |
| cg27519140 | RPP21 | 6 | 88.7 | −0.005 | 1 × 10−11 | −0.005 | 0.04 |
| cg00422488 | ANP32B | 9 | 2.7 | −0.013 | 2 × 10−11 | −0.011 | 3 × 10−3 |
| cg05288253 | ANKRD11 | 16 | 70.2 | −0.004 | 1 × 10−10 | −0.003 | 0.03 |
| cg13740985 | PSAT1 | 9 | 33.5 | 0.004 | 2 × 10−9 | 0.003 | 0.02 |
| cg11440375 | DKFZP686I152 | 6 | 81.1 | −0.003 | 5 × 10−9 | −0.003 | 0.03 |
| cg02711608 | SLC1A5 | 19 | 18.1 | −0.008 | 1 × 10−8 | −0.005 | 0.04 |
| cg27637303 | N/A | 2 | 71.3 | −0.009 | 2 × 10−8 | −0.018 | 3 × 10−6 |
| cg16260349 | TSPAN14 | 10 | 65.7 | −0.005 | 3 × 10−8 | −0.006 | 5 × 10−3 |
| cg17466510 | N/A | 1 | 77.2 | −0.004 | 3 × 10−8 | −0.01 | 8 × 10−4 |
| cg18763536 | ETV6 | 12 | 34.6 | −0.004 | 4 × 10−8 | −0.007 | 3 × 10−4 |
| cg22994830 | PRKAR1B | 7 | 25.2 | 0.009 | 5 × 10−8 | 0.01 | 7 × 10−3 |
| cg03217115 | N/A | 2 | 61.9 | −0.006 | 8 × 10−8 | −0.013 | 4 × 10−6 |
| cg06983052 | LRRC8D | 1 | 69.5 | −0.004 | 8 × 10−8 | −0.01 | 5 × 10−3 |
Abbreviations: ANKRD11, ankyrin repeat domain 11; ANP32B, acidic leucine-rich nuclear phosphoprotein 32 family member b; cAMP, cyclic adenosine monophosphate; CpG, cytosine-phosphate-guanine; DKFZP686I152, hypothetical protein DKFZP686I152; ETV6, ETS variant 6; LRRC8D, leucine-rich repeat containing 8 VRAC subunit D; N/A, not applicable; PHGDH, phosphoglycerate dehydrogenase; PRKAR1B, protein kinase cAMP-dependent type 1 regulatory subunit β; PSAT1, phosphoserine aminotransferase 1; RPP21, ribonuclease p subunit 21; SLC1A5, solute carrier family 1 member 5; SLC43A1, solute carrier family 43 member 1; SLC7A11, solute carrier family 7 member 11; TSPAN14, tetrespanin 14.
a Association point estimates are presented as the difference in average percent methylation associated with an increase of 1 drink per day (7 drinks per week).