Fig. 4.

a Substrate specificity of An-lipase from A. niger F0215 to various p-nitrophenyl esters was examined using the substrates p-nitrophenyl acetate (C2), p-nitrophenyl butyrate (C4), p-nitrophenyl caprylate (C8), p-nitrophenyl laurate (C12) and p-nitrophenyl palmitate (C16) in phosphate buffer (pH 7.0) at 37 °C for 10 min. b Lineweaver–Burke plot of the inverse of An-lipase activity and the inverse of p-nitrophenyl caprylate concentration yielded K_m and V_max of 26.7 mmol/L and 129.9 mmol/(L h), respectively