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Purification of the recombinant An-lipase from A. niger F0215
| Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg protein) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Crude enzyme solution | 77.5 | 2542 | 32.8 | 1.0 | 100 |
| Ammonium sulfate precipitation | 3.2 | 1679 | 525 | 16.0 | 66.1 |
| Sephadex G-75 | 0.7 | 905 | 1293 | 39.4 | 35.6 |
