Fig. 2.

Hydrogen peroxide-induced changes in protein expression and cysteine thiol oxidation/reduction in 32 h after H₂O₂-treatment. (A) Correlation of protein expression between two biological replicates in 32 h time-interval is shown. The most significant protein expression changes (one-sample t-test, Benjamini-Hochberg FDR < 0.05, |log2FC| > 2SD) were marked with blue (downregulated) or red (upregulated) dots. Confirmation of expression changes of IL-1β (B) and MCM3 (C) using immunoblotting. Mean and standard deviation from three independent biological replicates are shown. Protein expression was normalized on corresponding loading control (GAPDH). Statistical significance was calculated by two-sided unpaired t-test, ** p-value < 0.01, * p-value < 0.05. (D) Global increase of cysteine oxidation was detected 32 h after H₂O₂-treatment. Correlation between two biological replicates in 32-h time-interval is shown. Hydrogen peroxide-sensitive cysteine peptides significantly upregulated in both biological replicates (two-sample t-test, permutation-based FDR < 0.05) were marked with red dots. (E) The most significant examples of hydrogen peroxide-sensitive proteins are shown (paired t-test p-value < 0.02, mean difference > 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)