Fig. 2.

(A) Representation of compound distribution by % inhibition of L. donovani axenic amastigote growth in the primary screen. Inactive and active hit compounds are color-coded gray and orange, respectively. (B) Distribution of the hits selected from primary screen according to InMac assay results during single point confirmation phase. Amastigote-specific activity (y-axis; on-target effect) is plotted against the measured % inhibition of host macrophage cells (x-axis; off-target effect). Those compounds that were progressed (orange) or deprioritized for a lack of activity and/or selectivity (gray) are color-coded. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)