Fig. 3.

(A) Representation of compound distribution by % inhibition of T. cruzi growth measured in the β-galactosidase assay used for the primary screen. Inactive and active hit compounds are color-coded gray and blue, respectively. (B) Distribution of the hits selected from primary screen according to high-content imaging and 72 h trypomastigote assays results during single point confirmation phase. Those compounds that were progressed (blue) or deprioritized for a lack of activity against trypomastigote (≤20% inhibition) (gray) are color-coded. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)