Figure 1.
Screening for a Factor to Enhance the Regenerative Capability of hUCB-MSCs
(A) Scheme of comparison of the different gene expression profiles of the effective hUCB-MSCs, M02, and the ineffective ones, M01. Scatterplot of whole gene expression profiles of M02 and M01 from our previous report.2 Screened seven genes represented according to expression level of M02 relative to M01 are shown. First, surface and secretory molecules were selected and their statistically significant differences in expression were identified (p ≤ 0.05). Among them, we selected secretory factors, EDN1and CXCL12, for application as priming factors. (B) Expression of EDN1 and CXCL12 mRNA were confirmed using qRT-PCR. EDN1 mRNA showed significantly higher expression in the effective clone of hUCB-MSCs than in the ineffective one. The levels of CXCL12 mRNA did not change significantly. (C) ELISA showed higher EDN1 secretion from the effective clone.