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. 2019 May 17;13:503–511. doi: 10.1016/j.omtm.2019.05.003

Figure 2.

Figure 2

EDN1 Upregulates Expression of CDH2 and VEGF

(A) qRT-PCR to determine the levels of CDH2 and VEGF mRNA. Transcription of CDH2 and VEGF increased with 0.025 μg/mL EDN1 compared with 0 and 0.25 μg/mL EDN1. (B–D) Optimization of EDN1 treatment time. (B) qRT-PCR to determine the levels of CDH2 is shown. Expression of CDH2 mRNA peaked at 6 h. (C) qRT-PCR to determine the levels of VEGF is shown. VEGF expression continues to increase from 1 to 72 h following EDN1 treatment. (D) ELISA to determine the levels of VEGF protein is shown. Secreted VEGF increased from 6 h and peaked at 24∼72 h. Taken together, the optimal condition of EDN1 treatment of cells is 0.025 μg/mL for 24 h.