Figure 1. Pten is required for receptor editing.

1. Representative analysis of B220 and IgM surface expression in bone marrow (left) and spleen cells (right) of mice from the indicated genotypes.
2. Representative flow cytometric analysis of splenocytes from mice of the indicated genotypes (pre‐gated on B cells: B220⁺/CD19⁺) for surface expression of IgM and the 3‐83 idiotype (54.1). Shown data are representative of 11–35 individual mice per genotype.
3. PCR fragments amplified with specific primers for Vκ and Jκ5 from genomic DNA of purified splenic B cells from Pten ^f/f × 3‐83 ^ki and Pten ^f/f × mb1‐cre × 3‐83 ^ki mice on the respective backgrounds. Genomic tail DNA from a 3‐83 ^ki mouse and DNA from purified splenic B cells of a control (ctrl) mouse were used as controls. PCR for the splicing factor Srp20 served as a loading control. K^b and K^d indicate the respective background of the mice (H2‐K^b: +Ag).