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. 2019 Apr 23;38(11):e100368. doi: 10.15252/embj.2018100368

Figure EV4. Related to Fig 7. Slx5/Slx8 alleviates EUC1 overexpression toxicity by curbing ectopic Euc1 localization.

Figure EV4

  1. Western blot against Euc1 (and Dpm1 as loading control) to compare expression levels in WT and pGPD‐EUC1 cells. Asterisk denotes a non‐specific band.
  2. EUC1 overexpression leads to strong phenotypes or lethality in slx5∆ and slx8∆ cells. Individual cells from tetrads (arranged in vertical columns) were grown on YPD plates at 30°C for 3 days.
  3. ChIP‐chip tracks from regions showing additional EUC1‐binding signals upon EUC1‐overexpression (pGAL‐EUC1, 3 h induction). Data represent means from two independent replicates.
  4. Ubiquitin (ub‐K48) ChIP‐qPCR for the same samples as shown in Fig 7E. Data represent means ± SD (n = 3). A.U.: arbitrary units.
  5. Overexpression of SLX5/SLX8 leads to a reduction of aberrant Euc1 binding to non‐ub‐HS loci. ChIP‐qPCR analysis of selected strains from Fig 7F as indicated, after 3‐h galactose induction. Data represent means ± SD (n = 3). P‐values from Student's t‐tests for the indicated comparisons are shown.