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. 2019 Apr 23;38(11):e100368. doi: 10.15252/embj.2018100368

Figure 8. EUC1 shows genetic interactions with regulators of gene expression upon thermo‐ and DMSO stress.

Figure 8

  • A, B
    EUC1 displays negative genetic interactions with genes involved in general and specific transcriptional regulation (A), and in particular with members of the Rpd3L histone deacetylase (HDAC) complex (B). For (A–D), serial dilutions of the indicated strains were spotted and grown on YPD control or selective media plates, or under conditions as indicated. For (B), plates were incubated for 2 days (22°C, 30°C, 4% DMSO) or 3 days (37°C). Note that all strains used in (A–D) contained an extra copy of MED11, see Appendix Fig S10C–F for details.
  • C
    Plasmid‐borne EUC1 complements genetic interactions with Rpd3L subunits. Empty vector (–) or a plasmid encoding EUC1 with its endogenous promoter were transformed in the indicated strains and spotted on selective media.
  • D
    EUC1 and Rpd3S act in a common pathway which is redundant with Rpd3L. rco1∆ and eaf3∆ (Rpd3S) show similar phenotypes when paired with sds3∆ (Rpd3L) as euc1∆ and are epistatic with euc1∆.
  • E
    Graphic summary of EUC1 genetic interactions as tested in (A–D) and Appendix Fig S10. Arrows indicate negative genetic interactions upon cold (blue), heat (red), or DMSO stress (black), and dashed gray arrows indicate epistatic relationships.