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. 2019 Apr 23;38(11):e100368. doi: 10.15252/embj.2018100368

Figure 9. Euc1‐mediated ub‐hotspots and Slx5/Slx8 are crucial for a functional stress response under impaired gene expression control.

Figure 9

  1. Euc1 is recruited to ub‐HSs upon mild heat stress (37°C, 90 min). ChIP‐qPCR experiments with strains and conditions as indicated. Data represent means ± SD (n = 4), see Appendix Fig S11B for statistical analysis.
  2. The ability to form ub‐hotspots is crucial for endogenous EUC1 function. The genetic interaction of EUC1 with SDS3 (Rpd3L complex) was rescued with plasmid‐borne EUC1 alleles, and serial dilutions were spotted on selective media. (–) denotes empty vector. See also Appendix Fig S12A and B.
  3. SLX5 also shows negative genetic interactions with SDS3 upon heat stress. Serial dilutions of the indicated strains were spotted on YPD and incubated as indicated. See also Appendix Fig S12C and D. Note that all strains used in (A–C) contained an extra copy of MED11, see Appendix Fig S10C–F for details.