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. Author manuscript; available in PMC: 2019 Jun 3.
Published in final edited form as: Mol Cell. 2018 May 31;70(5):842–853.e7. doi: 10.1016/j.molcel.2018.04.023

Figure 3. Ductal Injection of Lentiviruses Overexpressing Satellite RNA Induces Tumor Formation in the Mammary Gland.

Figure 3.

We injected 1 × 106 virus particles in 10 μL of PBS and 0.2% of trypan blue with a Hamilton syringe into the duct of the number 4 mammary gland in a 6-to8-week-old female mouse according to the protocol described previously (Krause et al., 2013).

(A) Top: the ductal injection of lentiviruses into the number 4 mammary glands. Bottom left: the lentiviral vector for overexpressing satellite RNA. Bottom right: representative image of mammary glands or tumors harvested from mice 5 months after the injection.

(B) Kaplan-Meier survival curves in mice injected with lentivirus-overexpressing satellite RNAs or control RNA using a log rank (Mantel-Cox) test. p < 0.0001.

(C) H&E (top) and IHC staining (bottom) of tumor sections. Tumors resulted from the infection of satellite RNA-expressing lentiviruses.

(D) qRT-PCR analysis of tumors from mice injected with satellite RNA-expressing lentiviruses. Error bars represent SEM.

Scale bars, 100 μm. See also Table S2.