Fig. 2.

Rational design of a compact AAV-CRISPR-Cas9 system with CRISPReader. a Design of a positive feedback loop for expressing dCas9-VP64. The loop amplified the cellular dCas9-VP64-GFP signals generated from the basal transcription of the TATA box. b The relative GFP fluorescence at various time points was measured by FACS analysis. c Design and construction of the all-in-one AAV-dCas9 system. The Cas9/intron-RNA array gene is shown. d Schematic of the mammalian luciferase reporter system used to evaluate transactivation efficiency of the AAV-dCas9 system. e The results of the luciferase assay. Data are the mean ± SD from five experiments. **P < 0.01, compared with the negative control using the paired, one-sided t test. f, g The relative RNA levels of VEGF and MALAT1. Data are the mean ± SD from five experiments. **P < 0.01, compared with the negative control using the paired, one-sided t test