Fig. 3.

In vivo gene activation in hypercholesterolemic mice with the all-in-one AAV system. a Flowchart showing the key steps of AAV dCas9-mediated gene activation in the hypercholesterolemic mice model. b Design and construction of the all-in-one AAV-dCas9 system, as well as the two dual-AAV systems used as controls. c The relative mRNA levels of Apoa1 in AML12 cell line. Data are the mean ± SD from five experiments. **P < 0.01, compared with the negative control, determined with a paired, one-sided t test. d Time courses of plasma levels of Apoa1, HDL, and TC in four groups (n = 5 for each group) of AAV-treated hypercholesterolemic mice. Data are expressed as the mean ± SD. e The relative mRNA levels of Apoa1 in mice liver tissues. Data are the mean ± SD from five experiments. **P < 0.01, compared with the negative control, determined with a paired, one-sided t test