Figure EV1. TRF2 overexpression in cancer cells induces a strong immunosuppressive microenvironment via the recruitment of MDSCs and regulatory T cells.

• A–D
  Analysis of immune cell infiltration in TRF2‐overexpressing or TRF2‐knockdown B16F10 melanoma tumors. Box plots of total immune cell infiltration (CD45⁺ cells), total T cell infiltration (CD45⁺ CD3⁺ T cells), and CD8⁺ or CD4⁺ T cells among CD3⁺ cells infiltrating (A). Representative density plot of regulatory T cells (CD25⁺ Foxp3⁺ Treg) and activated T cells (CD25⁺ Foxp3⁻) (B). Box plots of infiltrating Treg (CD25⁺ Foxp3⁺) and activated T cells (CD25⁺ Foxp3⁻) (C) or NK cells (NKp46⁺ CD3⁻) (D).
• E
  Analysis of MDSC infiltration in TRF2‐overexpressing or TRF2‐knockdown BJcl2 tumors in Nude mice.
• F, G
  Data used for the correlation analysis presented in Fig 1F–I. Box plots present the percentages of CD11b^Hi GR1^Hi MDSCs among CD45⁺ cells infiltrating the Matrigel plug, the mean fluorescence intensity (MFI) of intranuclear pSTAT3 staining in MDSCs, the percentages of intratumoral CD3⁻ NKp46⁺ NK cells among CD45⁺ cells, and NKp46⁺ IFN‐γ⁺ NK cells (F). The mRNA expression level (relative quantity) in the Matrigel plugs of arg1, tgfβ, and il‐10.

Data information: In (A–D), data are presented as box plots min to max showing all points with median from n = 5 or 10 tumor‐bearing mice per group (*P < 0.05, **P < 0.01, and ***P < 0.001, Mann–Whitney test). In (E), data are presented as box plots min to max showing all points with median from n = 8 tumor‐bearing mice per group. In (F–G), data are presented as box plots min to max showing all points with median from n = 8 mice per group of Matrigel plug assay (*P < 0.05, **P < 0.01, and ***P < 0.001, Mann–Whitney test).