Figure 1.

Marinobufagenin's effect on leukocyte migration to the peritoneal cavity. Swiss mice were pretreated for three consecutive days with marinobufagenin (0.56 mg/kg), saline, or DMSO. One hour after the last day of pretreatment, animals were stimulated with zymosan at 2 mg/mL concentration. Four hours after of zymosan stimulus, peritoneal exudate was collected and total and differential leukocyte numbers were evaluated. (a) Total cell number. (b) Polymorphonuclear cell number. (c) Mononuclear cell number. Numerical data were presented as mean ± e.p.m of two experiments with n = 5 and analyzed by one-way analysis of variance (ANOVA) followed by Tukey posttest. ^#p < 0.05, significant when compared to the saline group. ^∗p < 0.05, significant when compared to the zymosan group. DMSO: dimethyl sulfoxide; MBG: marinobufagenin; ZYM: zymosan.