Figure 6.

Marinobufagenin's effect on surface molecules' expression and p38 phosphorylation. Peritoneal macrophages were stimulated with zymosan (0.2 mg/mL) and treated with marinobufagenin concentrations 10 nM for 24 h. Cells were labeled separately with antibodies anti-TLR2, anti-CD69, and anti-P-p38 and analyzed by a flow cytometer. (a) Representative fluorescence histograms, (b) TLR2⁺ (%), (c) CD69⁺ (%), and (d) P-p38⁺ (%). Numerical data were presented as mean ± e.p.m of two experiments with n = 3 and analyzed by one-way analysis of variance (ANOVA) followed by Tukey posttest. ^#p < 0.05, significant in relation to the control group. ZYM: zymosan; MBG: marinobufagenin.