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. 2019 May 19;2019:2945435. doi: 10.1155/2019/2945435

Figure 4.

Figure 4

Expression of dopaminergic neuron-specific proteins. (a) Western blot of microtubule-associated protein 2 (MAP2) expression performed in triplicates for all cell lines, at Day -15 and Day 42. (b) Differential detection of synaptosome-associated protein 25 (SNAP25), microtubule-associated protein tau (MAPT), and alpha synuclein (SNCA) in undifferentiated and differentiated OE-MSCs via mass spectrometry. As shown here, these three proteins were not detected in undifferentiated cells but were produced by differentiated cells on Day 45. (c) Immunostaining of differentiated OE-MSCs (Day 42) with antibodies raised against tubulin beta 3 (TUBB3), forkhead box protein A2 (FOXA2), LIM homeobox transcription factor 1 alpha (LMX1A), nuclear receptor subfamily 4 group A member 2 (NR4A2), engrailed homeobox 1 (EN1), pituitary homeobox protein 3 (PITX3), potassium voltage-gated channel subfamily J member 6 (KCNJ6), calbindin (CALB, green), and MAP2 (red).