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Dopamine synthesis by differentiated OE-MSCs. (a) Western blot for tyrosine hydroxylase (TH), dopa decarboxylase (DDC), and solute carrier family 6 member 3 (SLC6A3). (b) Time-dependent expression of dopamine receptor D2 (DRD2), from Day -15 (no treatment) to differentiation Day 42. (c) Immunostaining at Day 42 with antibodies raised against TH, DDC, dopamine, SLC6A3, and DRD2. (d) Dopamine (DA) was quantified in cell lysates via high-performance liquid chromatography (HPLC).
