Table 1.
Antibodies used for immunostaining and western blot.
| Antibodies | Species | Concentration | Origin | Catalog number | |
|---|---|---|---|---|---|
| Primary antibodies | βIII-tubulin | Rabbit | IF: 1/1000 | Covance | PRB-435P |
| Calbindin | Rabbit | IF: 1/200 | Abcam | ab25085 | |
| Dopamine | Rabbit | IF: 1/500 | Abcam | ab6427 | |
| Dopa decarboxylase | Rabbit | WB: 1/1000 IF: 1/200 |
Abcam | ab3905 | |
| Dopamine D2 receptor | Goat | IF: 1/200 | Abcam | ab30743 | |
| Dopamine transporter | Rat | WB: 1/1000 IF: 1/200 |
Millipore | MAB369 | |
| Engrailed 1 | Rabbit | IF: 1/25 | Abcam | ab70993 | |
| Foxa2/HNF3b | Rabbit | WB: 1/1000 IF: 1/400 |
Cell Signaling | 8186 | |
| Girk2 | Goat | IF: 1/100 | Abcam | ab65096 | |
| Lmx1a | Rabbit | WB: 1/1000 IF: 1/200 |
Millipore | AB10533 | |
| MAP2 | Chicken | WB: 1/10000 IF: 1/5000 |
Abcam | ab5392 | |
| Nurr1 | Mouse | IF: 1/300 | Abcam | ab41917 | |
| Pitx3 | Rabbit | WB: 1/250 IF: 1/200 |
Abcam | ab30734 | |
| TH | Rabbit | WB: 1/500 | Millipore | AB152 | |
| TH | Rabbit | IF: 1/500 | Pel-Freez | P40101-150 | |
| Actin-HRP | Mouse | WB: 1/10000 | Proteintech | HRP-60008 | |
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| Secondary antibodies | Rabbit-HRP | Goat | WB: 1/6000 | Cell Signaling | 7074 |
| Chicken-HRP | Goat | WB: 1/6000 | Abcam | ab97135 | |
| Rat-HRP | Goat | WB: 1/5000 | Millipore | AP136P | |
| Anti-rabbit-Alexa 488 | Goat | IF: 1/1000 | Thermo Fisher | A11070 | |
| Anti-goat-Alexa 488 | Donkey | IF: 1/1000 | Thermo Fisher | A11055 | |
| Anti-mouse-Alexa 488 | Goat | IF: 1/1000 | Invitrogen | A11029 | |
| Anti-rat-Alexa 488 | Goat | IF: 1/1000 | Thermo Fisher | A11006 | |
| Anti-chicken-Alexa 647 | Goat | IF: 1/1000 | Thermo Fisher | A21449 | |
| HCS CellMask Orange | IF: 1/20000 | Thermo Fisher | H32713 | ||
| DAPI | IF: 1/10000 | Sigma Aldrich | D8417 | ||
WB: western blot; IF: immunofluorescence; HCS: high-content screening.