Figure 7.

Reduced ability of DCs from Cpn-infected iNKT KO mice to induce activation and IFN-γ production by naïve NK cells. DCs were isolated from spleens of iNKT KO mice (KO-DC) and WT mice (WT-DC) at day 5 p.i. NK cells were enriched from the spleen of naïve mice. NK cells (2 × 10⁵) were cocultured with DC (1 × 10⁶) cells in a 96-well round bottom plate. After 48 h, the cells and supernatant were collected for CD69, CD25, and IFN-γ staining of the cells and ELISA measurement of IFN-γ in the supernatants, respectively. The representative histograms and dot plots for CD69 (a) and CD25 (b) expression and IFN-γ production (e) by NK cells are depicted. The percentage of CD69+ (b), CD25+ (d), and IFN-γ+ (f) NK cells was summarized. (g) The concentration of IFN-γ in supernatant was examined by ELISA. The data represent one of three similar experiments and are shown as mean ± SD. ^∗ p < 0.05, ^∗∗ p < 0.01.