Table 6.

Primers sequences used in simplex and multiplex PCR assays for virulence genes.

The gene	Primer	Amplification size and   Tm	References
chuA	F: 5′-GACGAACCAACGGTCAGGAT-3′   R: 5′-TGCCGCCAGTACCAAAGACA-3′	279 bp   (multiplex) 55°C	Clermont et al., 2000
yjaA	F: 5′-TGAAGTGTCAGGAGACGCTG-3′   R: 5′-ATGGAGAATGCGTTCCTCAAC-3′	211bp (multiplex) 55°C	Clermont et al., 2000
TspE4C2	F: 5′-GAGTAATGTCGGGGCATTCA-3′   R:5′-CGCGCCAACAAAGTATTACG-3′	152 bp (multiplex) 55°C	Clermont et al., 2000
hlyD	F: 5′- CTCCGGTACGTGAAAAGGAC-3′   R: 5′-GCCCTGATTACTGAAGCCTG-3′	904 bp   55°C	Rodrigues-Sike et al., 2005
kpsMTII	F: 5′- GCG CAT TTG CTG ATA CTG TTG-3′   R: 5′-CAT CAG ACG ATA AGC ATG AGC A-3′	272 bp   60°C	Johnson et al., 2005 [43]
hlyA	F:5′-AACAAGGATAAGCACTGT TCTGGC T-3′   R:5′-ACCATATAAGCGGTCATT CCC GTC A-3′	1,177 bp   60°C	Yamamoto et al., 1995 [44]
sfaD/E	F:5′-CGGAGGAGTAATTACAAACCTGGCA -3′   R: 5′- CTCCGGAGAACTGGGTG ATCTTA C-3′	408 bp   60°C	Blanco et al., 1997 [45]
fimH	F: 5′-TGC AGA ACG GAT AAG CCG TGG -3′   R: 5′- GCA GTC ACC TGC CCT CCG GTA -3′	508 bp   63°C	Rodrigues-Sike et al., 2005
afaBC	F: 5′-GCTGGGCAGCAAACTGATAACTCTC -3′   R:5′CATCAAGCTGTTTGTTCGTCCGCCG-3	793 bp   63°C	Blanco et al., 1997
cnf-1	F: 5′- GAA CTT ATT AAG GAT AGT-3′   R: 5′-CAT TAT TTA TAA CGC TG-3′	543kb   40°C	Blanco et al., 1997

Table 6 is reproduced from Grosu et al. 2017 [under the Creative Commons Attribution License/public domain].