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. 2019 Jan 24;104(6):1189–1201. doi: 10.3324/haematol.2018.202044

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Figure 1. — Characterization of transgenic human embryonic stem cells expressing the reciprocal fusion A4M together with MA4. (A) RNA-sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) validation revealed that ~45% (11/25) of the patients with t(4;11)⁺ B-cell precursor acute lymphoblastic leukemia do not express the reciprocal fusion A4M.¹⁸ (B) Left, Phase-contrast morphology of representative colonies from each transgenic human embryonic stem cell (hESC) line. Right, Reverse transcriptase polymerase chain reaction analysis (RT-PCR) confirming expression of both fusions in undifferentiated hESC. (C) qRT-PCR expression of the pluripotency genes OCT4, SOX2, NANOG, CRIPTO, and DNMT3B. (D) Representative FACS data confirming expression of the pluripotency surface markers SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81. BCP-ALL: B-cell precursor acute lymphoblastic leukemia; RNA-seq: RNA-sequencing; pos: positive; neg: negative; EV: empty vector; C+: positive control.