Skip to main content
. 2019 Mar 25;3(6):763–775. doi: 10.1002/hep4.1341

Figure 6.

Figure 6

Loss of LCN2 or treatment with recombinant LCN2 protein regulates lipolysis, FAO, and lipogenesis in hepatocytes. (A) Lcn2+/+ or Lcn2−/− mice were fed an HFCF diet for 16 weeks. Hepatic TGH activity was determined (n = 7‐8). (B) FAO was determined using [3H]palmitic acid as substrate in primary hepatocytes isolated from Lcn2+/+ mice or Lcn2−/− mice (n = 6). (C) mRNA levels were quantified in primary hepatocytes isolated from Lcn2+/+ mice or Lcn2−/− mice (n = 4). (D‐F) Primary hepatocytes were isolated from Lcn2+/+ or Lcn2−/− mice and then treated with phosphate‐buffered saline (control) or 500 ng/mL LCN2 for 30 hours (n = 4‐5 per group). TGH (D) and FAO (E) were determined. The mRNA levels of genes involved in lipogenesis, FAO, or lipolysis were determined. *P < 0.05, **P < 0.01. Abbreviations: PBS, phosphate‐buffered saline.