Figure 5:

PGK1 activity in glycolysis and regulation by acetylation.

PGK1 function is regulated by acetylation at K323, which enhances PGK1 enzymatic activity, improves glycolytic metabolism and promotes tumor growth. K323 acetylation is regulated by PCAF and SIRT7, allowing stimulation or inhibition of PGK1 function. P300/CBP-associated factor (PCAF) is a transcriptional coactivator associated with p53 promoting acetylation of PGK1. Conversely, NAD-dependent deacetylase sirtuin 7 (SIRT7), a nuclear enzyme, suppressed DNA recombination. GAPDH: glyceraldehyde 3 phosphate dehydrogenase. PGK1: Phosphoglycerate kinase 1 (Adapted from [76]).