Figure 3. Validation of pivotal plane-based area calculations.

All bilayers were composed of 100% DMPC. Error bars represent 95% confidence intervals estimated from standard errors A) Relationship between interleaflet ratios and radii for spheres and vesicles. The red and blue dashed lines are theoretical contours to z_o=0.85 nm for cylinders and spheres, respectively. x and y error bars are smaller than markers. B) Calculated z_o locations after equilibration for vesicles and cylinders. The dotted line is set at z_o = 0.85 nm, with grey shading to indicate 95% confidence interval reported by Wang and Deserno. C) Time-course of observed z_os for 10 nm radius cylinders created with zⁱ_o parameters of 0.0 nm and 2.0 nm, equilibrated with 2.0 nm radius pores.