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. 2019 May 27;10:1075. doi: 10.3389/fimmu.2019.01075

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Copyright © 2019 van Dalen, Blom, Walgreen, Slöetjes, Helsen, Geven, ter Huurne, Vogl, Roth, van de Loo, Koenders, Casteilla, van der Kraan, van den Bosch and van Lent.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Clustering of murine PMNs with ASCs is enhanced by IL-1β in vitro. The association of freshly isolated murine PMNs with adherent ASCs driven by IL-1β was assessed in a co-culture experiment. (A) After 6 h, clustering cells were stained with May-Grünwald Giemsa and quantified. Large cells are ASCs, small cells are PMNs. Both the number of ASCs that formed clusters with PMNs (B) and the average number of PMNs clustered per ASC (C) were significantly increased in presence of IL-1β when compared to the non-stimulated co-cultures (NS). N = 3 per group, quantified at five separate areas per sample. Differences between the groups were tested using a Student's t-test. Bars show mean values ± SD. *P < 0.05, **P < 0.01.