Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 27;10:1134. doi: 10.3389/fimmu.2019.01134

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Bouteau, Kervevan, Su, Zurawski, Contreras, Dereuddre-Bosquet, Le Grand, Zurawski, Cardinaud, Levy and Igyártó.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Steady state LCs, but not cDC1s can support the formation of GC-dependent antibody responses; cDC1s prevent LCs from inducing humoral immune responses. Mice were immunized with 1 μg of 4C7 (hIgG4 core) through the indicated DC subsets and the anti-hIgG4 responses induced were assessed 14 days later by: (A) ELISA (serum), (B) ELISpot (LNs), (C) Flow cytometry (LNs, % of hIgG4-specific B cells), (D) Flow cytometry % GC B cells (CD38⁺GL7⁻), and (E) histology. Skin draining LNs harvested from mice immunized through LCs or cDC1s were harvested at day 14 and stained with PNA to highlight GCs. Each dot represents a separate mouse. Data from at least 3 different experiments were pooled. ***p < 0.001, ****p < 0.0001, ns = not significant.