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. 2019 Jun 1;33(11-12):705–717. doi: 10.1101/gad.322453.118

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© 2019 Jiang et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — The RING domain of Not4 is required for stress tolerance and normal Rpb1 degradation. (A) Spot growth assays. Strains were spotted on YPD plates with the treatments indicated. The images of growth at 37°C with 60 J/m² UV and 100 mM HU were taken after 2 d. (B) Western blot of extracts from wild-type and mutant strains. The experiment was conducted as described in the legend for Figure 1A, except that cells were treated for 60 min. Asterisks mark nonspecific bands reacting with the Not4 antiserum. (C) Spot growth assays, as in A. (D) Western blotting of extracts from ΔRING and ΔRRM (RNA recognition motif) mutant strains.