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. 2019 Jun 1;33(11-12):626–640. doi: 10.1101/gad.324467.119

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© 2019 Li et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — The bHLH domain of Twist2 is critical for repressing myogenesis. (A) Sequence alignment of Twist2 and MyoD bHLH domains. The asterisk represents identical amino acids. (B) Chimeric Twist2 and MyoD bHLH constructs. Numbers represent amino acid number. (C) Experimental scheme to test the effect of Twist2 and MyoD chimeras on differentiation. (D) Tw2⁺ myoblasts infected with GFP, MyoD, Twist2, Twist2(M), or MyoD(T) were differentiated for 4 d and subsequently fixed and stained for Myosin (My32; green), tdTomato (red), and nuclei (blue). Scale bar, 50 µm. (E) The number of differentiated cells was quantified as a differentiation index based on the percentage of nuclei within My32⁺ cells compared with total nuclei. Unpaired two-tailed t-test: (****) P < 0.00005.