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. Author manuscript; available in PMC: 2019 Jun 3.
Published in final edited form as: Exp Cell Res. 2017 Oct 7;361(1):101–111. doi: 10.1016/j.yexcr.2017.10.007

Fig. 4. EECD34 cells maintain Pitx2 expression in vitro.

Fig. 4.

Representative images of EECD34 cells from wild type mouse EOM after (A) 24 h, (B) 48 h, or (C) 72 h in vitro in proliferation media immunostained for Pitx2 (green) and DAPI (blue). The bottom cell in (A) is dividing, and both are Pitx2-positive. Horizontal arrows indicate Pitx2 positive cells. Vertical arrow indicates a cell negative for Pitx2 (C). Scale bar is 30 μm. (D) Percentage of EECD34 cells from wild-type mouse EOM positive for Pitx2 after 24, 48, 72, and 96 h in vitro.