. Author manuscript; available in PMC: 2020 Apr 24.

Published in final edited form as: J Am Chem Soc. 2019 Apr 12;141(16):6631–6638. doi: 10.1021/jacs.9b01261

Table 1.

Properties of carboVoltageFluor indicators

compound	X	R²	λmax / nm^a	λem / nm^a	Φ^b	ΔF/F^c,^d	relative brightness^d	SNR^d,^e
carboVF2.1.H (26)	H	H	552	576	0.015	3 ± 1%	0.27 ± 0.03	2.2 ± 0.4
carboVF2.1(OMe).H (27)	H	OMe	554	576	0.037	12 ± 1%	0.58 ± 0.07	21 ± 3
carboVF2.1.F (28)	F	H	562	593	0.018	6 ± 0.4%	0.84 ± 0.03	19 ± 2
carboVF2.1(OMe).F (29)	F	OMe	562	593	0.009	26 ± 2%	0.93 ± 0.04	63 ± 10
carboVF2.1.Cl (30)	Cl	H	562	593	0.008	7 ± 1%	0.33 ± 0.01	43 ± 8
carboVF2.1(OMe).Cl (31)	Cl	OMe	562	593	0.012	31 ± 2%	1.00 ± 0.05	171 ± 10

Determined in HBSS with 0.01% SDS.

Determined in HBSS.

Per 100 mV.

Determined in HEK cells.

Sampled at 500 Hz. Error is ± S.E.M. for n = at least 5 cells for ΔF/F and SNR determination and n = 3 coverslips (>100 cells per coverslip) for relative brightness.