TABLE 2.
Body weight and plasma hormones of pups in CTR, HIGH n−3, and LOW n−6 groups at PNs 5, 10, 12, 14, 16, 21, 28, and 981
| CTR | HIGH n−3 | LOW n−6 | ||||
| Mean ± SEM | n [litters (pups)] | Mean ± SEM | n [litters (pups)] | Mean ± SEM | n [litters (pups)] | |
| Body weight (g) | ||||||
| PN | ||||||
| 5 | 2.8 ± 0.3 | 3 (9) | 2.8 ± 0.3 | 3 (8) | 2.7 ± 0.3 | 3 (10) |
| 10 | 4.9 ± 0.3 | 3 (9) | 5.3 ± 0.3 | 3 (9) | 5.1 ± 0.3 | 3 (9) |
| 12 | 6.4 ± 0.3 | 3 (9) | 6.8 ± 0.3 | 3 (9) | 6.1 ± 0.3 | 3 (9) |
| 14 | 6.8 ± 0.3 | 3 (9) | 7.6 ± 0.3 | 3 (8) | 7.0 ± 0.3 | 3 (9) |
| 16 | 8.0 ± 0.3 | 3 (8) | 7.8 ± 0.3 | 3 (9) | 7.1 ± 0.3 | 3 (9) |
| 21 | 8.6 ± 0.3 | 3 (9) | 10.2 ± 0.3 | 3 (9) | 9.1 ± 0.3 | 3 (9) |
| 28 | 15.9 ± 0.4 | 4 (14) | 16.1 ± 0.5 | 3 (12) | 16.6 ± 0.5 | 3 (11) |
| 98 | 32.7 ± 0.8 | 3 (12) | 32.3 ± 0.8 | 3 (12) | 32.5 ± 0.8 | 3 (11) |
| Leptin (ng/mL) | ||||||
| PN | ||||||
| 5 | 4.9 ± 1.5 | 3 (9) | 5.3 ± 1.6 | 3 (7) | 3.6 ± 1.4 | 3 (10) |
| 10 | 10.4 ± 1.5 | 3 (9) | 11.4 ± 1.5 | 3 (8) | 9.4 ± 1.5 | 3 (8) |
| 12 | 12.7 ± 1.5 | 3 (8) | 13.4 ± 1.5 | 3 (9) | 14.9 ± 1.5 | 3 (9) |
| 14 | 8.1 ± 1.5 | 3 (9) | 7.4 ± 1.5 | 3 (8) | 8.5 ± 1.5 | 3 (9) |
| 16 | 5.9 ± 1.5 | 3 (8) | 6.0 ± 1.5 | 3 (9) | 7.4 ± 1.5 | 3 (9) |
| 21 | 3.5 ± 1.5 | 3 (9) | 3.2 ± 1.5 | 3 (8) | 4.4 ± 1.5 | 3 (8) |
| Ghrelin (ng/mL) | ||||||
| PN | ||||||
| 16 | 0.43 ± 0.07 | 3 (8) | 0.36 ± 0.07 | 3 (8) | 0.30 ± 0.07 | 3 (7) |
| 21 | 0.21 ± 0.07 | 3 (8) | 0.22 ± 0.07 | 3 (7) | 0.21 ± 0.07 | 3 (8) |
1
Data from each sample time represent a different set of animals. Significances between groups were determined by using a mixed-effects regression model followed by Tukey-Kramer post hoc tests. There were no significant differences between groups in body weight and plasma hormones. CTR, control diet; HIGH n−3, diet with a relative increase in n−3 fatty acid; LOW n−6, diet with relative reduction in n−6 fatty acid; PN, postnatal day.