Table 3. Study design.
| Study Group | Dosage Level (X of targeted dose) |
Target Dosage Level (% of AOCED in the diet on DM basis) |
Actual DHA+EPA content* (% in the diet As Is) |
DHA+EPA content (% in the diet on DM basis) ** |
Number of Animals | ||
|---|---|---|---|---|---|---|---|
| F0 Generation (dams)a |
F1 Generation (puppies)b |
||||||
| F | M(Spare) | F(Spare) | |||||
| Control | 0 | 0 | 0.11% | 0.12% | 5 | 4(1) | 4(1) |
| AOCED Low Dose | ½X | 0.75% | 0.41% | 0.44% | 5 | 4(1) | 4(1) |
| AOCED Mid Dose | 1X | 1.5% | 0.77% | 0.83% | 5 | 4(1) | 4(1) |
| AOCED High Dose | 2X | 3.0% | 1.55% | 1.69% | 5 | 4(1) | 4(1) |
* Values presented as a sum of DHA and EPA (Weight %) per Certificate of Analysis.
** Calculated values based on dry matter basis (adjusted for moisture content).
a Six females per group were mated to have at least 5 females per group confirmed pregnant at GD 29–35.
b Two puppies per litter (a male and a female) were randomly selected, the spare was randomly selected from any litter within each group.
M: male; F: female