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. 2019 May 21;15(5):e1007803. doi: 10.1371/journal.ppat.1007803

Fig 4. Characterization of SEA-binding mast cells.

Fig 4

Specimens of the intestinal tract were removed from a common marmoset. Frozen tissues were sectioned (10 μm) and incubated with SEA (1.0 μg/ml) for 60 min at room temperature. (A) Ileal sections were processed for double immunofluorescence staining using anti-SEA antibody (green) and anti-tryptase mAb (red), which recognizes the mast cell marker, tryptase. (B) Ileal sections were processed for double immunofluorescence staining using anti-FcεRIα antibody (green) and anti-5-HT antibody (red). (C) Ileal sections were processed for double immunofluorescence staining using anti-histamine antibody (green) and anti-FcεRIα antibody (red). Each scale bar is equal to 20 μm.