Fig 6. SEA induces the release of histamine from the GI tract.

Sections of common marmoset jejunum were incubated with or without SEA, and histamine and serotonin released from the jejunum were measured. (A) Jejunal sections were incubated with various concentrations of SEA for 2 h at 37°C in a CO₂ incubator and histamine in the culture supernatant fluid was measured using ELISA. (B) Jejunal sections were incubated with 100μg of SEA and various concentrations of DSCG for 2 h and histamine in the culture supernatant fluid was measured using ELISA. (C) Jejunal sections were incubated with various concentrations of SEA for 2 h and 5-HT in the culture supernatant fluid was measured using ELISA. (D) Jejunal sections were incubated with 100 μg of SEA and various concentrations of DSCG for 2 h and 5-HT in the culture supernatant fluid was measured using ELISA. Each bar represents the mean ± SD from 8 jejunal pieces. The statistical analysis was performed using ANOVA followed by Tukey’s post hoc test, *P<0.05.