Figure 2.

Epithelial monolayer maturity impacts desmoglein-2 (DSG2) redistribution during EPEC infection. Young (A) and old (B) C2_BBe monolayers cultured up to 1-day or 7-days post-confluence, respectively, were infected with enteropathogenic Escherichia coli O127:H6 strain E2348/69 at a MOI = 100 for 3 hours. Cartoons depict expected distribution of DSG2 (magenta) and keratin intermediate filaments (green) in adjacent epithelial cells. Lower panels in (A) and (B) show immunofluorescence staining of DSG2 (magenta) and cytokeratin 8/18 (green) in uninfected or EPEC-infected young and old C2_BBe monolayers, respectively. DAPI (blue) was used to stain DNA. A broken white line outlines cell junctions.