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Response of the IPEC-J2 to different concentrations of toxin A (TcdA) and toxin B (TcdB) as measured by transepithelial electrical resistance (TEER) in an in vitro assay up to 20 h of incubation. Control: growth media (Dulbecco’s Modified Eagle’s Medium – DME, DMEM | Sigma-Aldrich). Spent supernatant containing TcdA (1 291 ng/ml) and TcdB (829 ng/ml) diluted 1:2, 1:10, 1:100 and 1:1 000. Methods in Supplementary file S1.
