Table 2.
Summary of various microbiome studies to highlight discrepancies in water sources and reporting.
| Author | Facility Type | Strain | Vendor | Water Source | Sex | Diet | LD Cycle | DOI/PMID |
|---|---|---|---|---|---|---|---|---|
| Van der Sluis et al.2 | SPF | 129Sv-Muc2−/− 129Sv-Muc2+/+ 129Sv-Muc2+/− | 129Sv acquired from Charles River Muc2−/− raised in house | Acidified tap water (pH not specified) [administered prior to experiment] Autoclaved tap water [administered during DSS exposure] | Male | Standard rodent pellets (Special Diet Services, Witham, Essex, England) given ad libitum | 12:12 LD | 10.1053/j.gastro.2006.04.020 |
| Burger-van Paassen et al.3 | SPF | 129Sv-Muc2−/− 129Sv-Muc2+/+ | 129Sv acquired from Charles River Muc2−/− raised in house | Acidified tap water (pH not specified) | Male | Standard rodent pellets (Special Diet Services, Witham, Essex, England) given ad libitum | 12:12 LD | 10.1371/journal.pone.0038798 |
| Lu et al.4 | SPF | 129Sv-Muc2−/− 129Sv-Muc2+/+ | 129Sv acquired from Charles River Muc2−/− raised in house | Acidified tap water (pH not specified) | Not specified | Standard rodent pellets (Special Diet Services, Witham, Essex, England) given ad libitum | 12:12 LD | 10.1002/ibd.21592 |
| Morampudi et al.5 | SPF | C57BL/6-Muc2−/− C57BL/6-Retnlb−/− C57BL/6 C57BL/6-Muc2−/−/Retnlb−/− | All mice raised in house | Autoclaved water (source not specified) | Not specified | Autoclave food (type not specified) | Not Specified | 10.1038/mi.2015.140 |
| Wolf et al.6 | SPF | NOD/ShiLtJ | NOD/ShiLtJ were acquired from JAX | Original breeder pairs received Birmingham city water which had been chlorinated and autoclaved Animals were then split between neutral pH (pH ~7.0) and acidified water (pH ~3.2) via the addition of HCL | Not specified | Autoclaved N1H-31 rodent diet (Harlan Teklan, Madison, WI) given ad libitum | Not Specified | 10.1369/0022155413519650 |
| Sofi et al.7 | SPF | NOD/ShiLtJ C57BL/6 | NOD/ShiLtJ were acquired from JAX or acquired in-house from a SPF colony of NOD/ShiLtJ Original C57BL/6 mice were purchased from Taconic Farms | Mice were maintained on either autoclaved neutral (pH7.0–7.2) or acidic (pH 3.0–3.2) water Water was acidified using HCL as per JAX instructions (http://jaxmice.jax.org/genetichealth/health_program.html; http://craniofacial.jax.org/new_standard_protocols.html |
Female | Not specified | Not Specified | 10.2337/db13-0981 |
| Graham et al.8 | SPF | Fox1nu/Fox1nu (Nude mice) | Animals were acquired from three vendors: Jax, Charles River and Taconic Farms | Not specified | Male | Irradiated Rodent Diet (Diet 2919 Teklad Global 19% protein rodent diet, Harland Laboratories) provided ad libitum | 12:12 LD | PMC3155402 |
| Brown et al.9 | SPF | NOD/ShiLtJ NOR/LtJ | Animals were acquired from JAX | Filtered, UV sterilized water | Female | Sterile chow (Laboratory Rodent Diet 5001, Purina Mills) ad libitum | 12:12 LD | http://doi.org/10.1038/ismej.2015.114 |