Figure 2.

crRNA abundance in M. mazei cas6b deletion strains.

Total RNA was isolated from the M. mazei wild type strain and the deletion strains ∆cas6b-IB and ∆cas6b-IIIC growing under standard and high salt conditions (500 mM NaCl) as described in the Materials and Methods. Subsequent northern blot analysis was performed, using a 5ʹ radioactively labeled subtype IB-repeat probe. In addition, the expression of 5S rRNA of the respective RNA preparation is shown. The pUC mix8 standard marker (Thermo Scientific) was used (fragment sizes are indicated at the left side). On the right side, observed crRNA processing is depicted schematically.