Figure 3.

crRNA processing in M. mazei wt and cas6b deletion strains for CRISPR-Cas subtypes I-B and III-C analyzed by an RNA-Seq approach.

Normalized sequencing reads (HiSeq) for the CRISPR array subtype I-B (a) and subtype III-C (b) regions are mapped to the M. mazei genome. Comparison of analyzed RNA-Seq reads derived from exponentially growing cultures from wt and respective M. mazei deletion strains ∆cas6b-IB and ∆cas6b-IIIC. Cells were grown under high salt conditions (500 mM NaCl) and total RNA was treated with T4 polynucleotide kinase (PNK) as described in Materials and Methods.