Figure 4.

crRNA 5ʹ and 3ʹ end processing on single nucleotide level in the M. mazei cas6b deletion strains.

Normalized RNA-Seq reads for the CRISPR array subtype I-B and subtype III-C are mapped to the M. mazei genome. Comparison of analyzed RNA-Seq reads derived from the wt and from the M. mazei deletion strains ∆cas6b-IB and ∆cas6b-IIIC. Cells were grown under high salt conditions (500 mM NaCl) as described in Materials and Methods. Depicted is the crRNA containing spacer 4 from the subtype-IB array and the crRNA for the spacer 8 belonging to the subtype-IIIC array. The crRNA processing is shown on single nucleotide level, with one bar representing a single nucleotide. The position of spacer (grey box) and repeat (dark grey line) sequences are indicated according to the RNA-Seq reads.