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. 2018 Aug 10;16(4):397–403. doi: 10.1080/15476286.2018.1493329

Figure 2.

Figure 2.

Modification of the malA gene with CRISPR-Cas9 in U. trichophora by using the vectors and malAg#2. .(a) The two target sites of malAg#1 and malAg#2 with their corresponding protospacer adjacent motifs (PAMs) in the malA gene are shown. The predicted Cas9-cleavage site 3-bp upstream of PAM is indicated. (b) Sequences of the targeted malA region from 13 transformants of pMF-hCas9-PU6-Tri-malAg#1 (numbered with 1–13) and 12 transformants of the control vector pMF-hCas9-U6-tri-scaff (numbered with c1-c12) are aligned to the wild-type malA sequence. The vertical line indicates the predicted CRISPR-Cas9 induced DSBs. Clone 10 (indicated with an asterisk) contained wild-type malA sequence. (c) Sequences of the targeted malA region from 4 analyzed transformants of pMF-hCas9-PU6-Tri-malAg#2 are aligned to the wild-type malA sequence.