Figure 1.

Extracellular miRNA stability at physiological temperature. (a) C2C12 myotube conditioned medium was incubated at 37ºC and miRNA abundance determined at each time point by RT-qPCR. (b) Table of miRNA half-lives and goodness-of-fit (R²) values for each miRNA in C2C12 myotube conditioned medium. (c) Serum from dystrophic mdx mice was incubated at 37ºC as indicated and miRNA abundance determined at each time point. (d) Table of miRNA half-lives and goodness-of-fit (R²) values for each miRNA in dystrophic serum. Curves were fitted by non-linear regression using a one phase exponential decay model. Values are mean±SEM, n = 2. LOQ, limit of quantification.