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. 2019 Mar 23;16(6):770–784. doi: 10.1080/15476286.2019.1585738

Figure 2.

Figure 2.

miR-1246-like sequences are highly enriched in PANC-1 exosomes. (a and b), qRT-PCR analysis using the poly A tailing method on miR-1246 expression in hTERT-HPNE, PANC-1 and MIA-PaCa-2 cells and their exosomes (means ± SEM, n = 3). The melting curves showed detection of different fragments in cells versus exosomes with the same set of primers. (c), Representative sequencing results from three individual clones of the detected exosomal miR-1246 and cellular RUN2-1. (d), miR-1246 target gene reporter assay. Mimics of mature miR-1246 and the exosomal miR-1246 variant suppress reporter gene activity as compared to scramble mimics (means ± SEM, n = 3). * p < 0.05, one-way ANOVA, followed by Dunnett analysis. (e), Northern blot analysis using a labeled miR-1246 probe against RNAs from several human cancer cell lines. There was no precursor miR-1246 and mature miR-1246 being detected and the primary band migrating at a size of RNU2-1 in all cell lines examined.