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. 2019 Mar 23;16(6):770–784. doi: 10.1080/15476286.2019.1585738

Figure 3.

Figure 3.

Effects of the MIR1246 gene knockout on exosomal miR-1246 expression. (a), The strategy of CRISPR-Cas9 HDR knockout of the MIR1246 gene. Ensembl gene ID and the precise sequence location are indicated. (b), GFP detection of the homozygous MIR1246 knockout PANC-1 cells by fluorescence microscopy. (c), Agarose gel electrophoresis of the PCR products of DNA from wild type PANC-1 and the MIR1246 knockout PANC-1 cells, using primers covering the DNA regions upstream and downstream of the MIR1246 gene. (d), qRT-PCR analysis using the poly A tailing method detecting miR-1246 in exosomes derived from wild type and MIR1246 knockout PANC-1 cells (means ± SEM, n = 3).