Figure 3.

Phage and host dynamics when both WT host and phage are diluted or bottlenecked. A 10⁻⁴ or 10⁻⁶ bottleneck corresponds to either 0.6 µl or 0.006 µl culture, respectively, in 6 ml of fresh media at each transfer. A 10^–4 dilution corresponds to 0.6 µl culture in 6 ml at each transfer, and a 10⁻⁶ dilution corresponds to 0.6 µl culture in 600 ml at each transfer (see Materials & methods). Mean plaque-forming units (p.f.u.) ml^–1 and colony-forming units (c.f.u.) for the dilution and comparable bottleneck treatments are shown. The detection limit is 200 p.f.u. or c.f.u. ml⁻¹. Error bars correspond to 95% confidence intervals (CIs). N = 6 for all treatments.