Figure 3.

HuR regulates cytokine production in Jurkat cells. (a) RNAseq mRNA level log₂ fold changes (FC) of expressed cytokines in activated KO1 vs. WT Jurkat cells. (b) Secreted IL-2 levels in WT and HuR KO cells after stimulation with PMA/ionomycin, n = 3. (c) Rescue of IL-2 production in HuR KO1 cells transduced with MSCV-MPIG-HuR retrovirus, compared to empty vector retrovirus, n = 3. Error bars represent standard error of the mean. *, p-value < 0.05, paired two-tailed Student’s t test of normalized ELISA spectrometry measurements.